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Video talks
on 16S data analysis posted.
URMAP
ultra-fast read mapper posted (
paper)
.
~20% of taxonomy annotations in SILVA and Greengenes are wrong (
paper
).
Taxonomy prediction is <50% accurate for 16S V4 sequences (
paper
).
97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (
paper
).
USEARCH
Download 32-bit
Buy 64-bit
New in v11
Tech support
Documentation
Commands
Topics
OTU analysis
Sequence search
Sequence clustering
Taxonomy
Diversity
Octave plots
Machine learning
Chimeras
Read quality
Paired reads
OTU errors and biases
Publications
Read quality filtering
Quality filtering overview
Read quality filtering
Expected error filtering
Discarding singletons
Calculating average Phred (Q) scores is a bad idea
Choosing filter parameters
Setting the expected error threshold for long reads
Defining unique sequence abundances
Filtering by merged